Medical University of South Carolina Hospital logo
Home |  Video Library | Podcast Library | e-Newsletters | Classes & Events | About Us | Community Blog | University & Colleges 
Contact Us | 843-792-1414
  

Patients & Visitors

Medical Services

Maps & Parking

Health Library

Physician Portal

Careers

Online Services
Health Library
Health Topics A to Z
Clinical Trials & Research
Drug Information
Tests & Procedures
Lab Tests & Results
Health Assessment Tools
Treatment Options
Symptom Checker
Health e-Newsletters
Podcast Library
Video Library
Health Library
Bookmark Page icon Bookmark |

Print this page icon

|

E-mail icon

Health Library : Lab Tests & Results Print [PDF]
Browse by the First Letter(s) of the Lab Test
ABCDEFGHIKLMNOPQRSTUVW

Borrelia burgdorferi DNA assay

What is this test?

This test detects the DNA of a specific parasite called Borrelia burgdorferi. A sample of blood, cerebrospinal fluid, synovial fluid, urine, or a skin sample may be collected for this test. This test is used when Lyme disease is suspected[1][2][3][4].

What are other names for this test?

  • Borrelia burgdorferi PCR
  • Lyme disease DNA detection

What are related tests?

  • Borrelia antibody assay
  • Lyme ELISA test
  • Lyme immunoblot test

Why do I need this test?

Laboratory tests may be done for many reasons. Tests are performed for routine health screenings or if a disease or toxicity is suspected. Lab tests may be used to determine if a medical condition is improving or worsening. Lab tests may also be used to measure the success or failure of a medication or treatment plan. Lab tests may be ordered for professional or legal reasons. The following is a possible reason why this test may be done:

  • Lyme disease

When and how often should I have this test?

When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear.

Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body.

The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may also need frequent monitoring. Certain tests may be repeated to obtain a series of results, or tests may need to be repeated to confirm or disprove results. Timing and frequency of lab tests may vary if they are performed for professional or legal reasons.

How should I get ready for the test?

Blood:

Before having blood collected, tell the person drawing your blood if you are allergic to latex. Tell the healthcare worker if you have a medical condition or are using a medication or supplement that causes excessive bleeding. Also tell the healthcare worker if you have felt nauseated, lightheaded, or have fainted while having blood drawn in the past.

Cerebrospinal fluid:

A lumbar puncture is a procedure that requires your written consent. Review the consent form with the healthcare worker and ask any questions that you have before signing the consent form. You may receive certain medications and need imaging tests done prior to the procedure.

Tell the person doing the lumbar puncture if you have a medical condition or are using a medication or supplement that causes excessive bleeding. Contact the healthcare worker if you have a history of chronic back pain, structural defects in your spine, or a past spinal surgery. Tell the healthcare worker if you have an infection on your back or if you have any psychiatric or neurological (nerve) conditions. You should also report if you have a history of allergic or other reactions to local anesthetics.

Synovial fluid:

An arthrocentesis is a procedure that requires written consent. Review the consent form with the healthcare worker and ask any questions that you have before signing the consent form.

Tell the healthcare worker if you have a medical condition or are using a medication or supplement that causes excessive bleeding. You should also report if you have a history of allergic or other reactions to local anesthetics. Blood tests may need to be done before an arthrocentesis.

Urine:

To prepare for giving a urine sample, be sure to drink enough fluids before the test, unless you have been given other instructions. Try not to empty your bladder before the test.

Other body fluid or tissue sample:

A different sample other than the samples listed above may be used for this test. Ask your healthcare worker for information about how to prepare for this test. If you have questions or concerns about the preparation for this test, talk to the healthcare worker.

How is the test done?

A sample of blood, cerebrospinal fluid, synovial fluid, urine, or a skin sample may be collected for this test[3][4].

Blood:

When a blood sample from a vein is needed, a vein in your arm is usually selected. A tourniquet (large rubber strap) may be secured above the vein. The skin over the vein will be cleaned, and a needle will be inserted. You will be asked to hold very still while your blood is collected. Blood will be collected into one or more tubes, and the tourniquet will be removed. When enough blood has been collected, the healthcare worker will take the needle out.

Cerebrospinal fluid:

Cerebrospinal fluid is the fluid that surrounds the brain and spinal cord tissues. The procedure that collects a sample of cerebrospinal fluid is called a lumbar puncture. For this procedure, you may need to lie on your side facing away from the person performing the procedure. You may be asked to roll up into a ball with your knees brought close to your chest. This position allows your spine to spread apart slightly and helps direct needle placement. This procedure may also be done while you are in an upright sitting position, with your knees drawn up toward your chest.

An area in the lower back will be chosen for the needle insertion site. This is about at the level of the top of your hip bone. Your skin will be cleaned with antiseptic and the area will be draped with sterile cloth. Local anesthetic will be injected with a small needle to numb the area. Anesthetic cream may also be applied. After the anesthetic has taken effect, the lumbar puncture needle is inserted between the vertebrae and into the spinal column. Cerebrospinal fluid is drawn out for testing and the needle is removed. You may need to change positions during the procedure if the healthcare worker is having difficulty collecting a sample of fluid. This procedure may be done more than one time if collecting cerebrospinal fluid is difficult.

Synovial fluid:

Synovial fluid is the fluid around the joints. A synovial fluid sample is obtained by a procedure called an arthrocentesis. The skin over the joint will be cleaned, and a local anesthetic will be used to numb the tissue at the insertion site. A needle will be inserted into the space around the joint, and fluid collected into a syringe. After enough synovial fluid has been collected, the needle will be removed.

Urine:

To provide a sample of urine, you will be asked to urinate into a container. Fill the container as much as you can, but do not overfill it. Urine samples may also be taken from a catheter.

Other body fluid or tissue sample:

A different sample other than the samples listed above may be used for this test. Methods used to collect other body fluids or tissue samples may vary. Ask the healthcare worker to explain how this sample may be collected. If you have questions or concerns about this test, talk to the healthcare worker.

How will the test feel?

The amount of discomfort you feel will depend on many factors, including your sensitivity to pain. Communicate how you are feeling with the person doing the procedure. Inform the person doing the procedure if you feel that you cannot continue with the procedure.

Blood:

During a blood draw, you may feel mild discomfort at the location where the blood sample is being collected.

Cerebrospinal fluid:

Before a lumbar puncture, a local anesthetic is given to the procedure site to numb the area. You may feel mild discomfort or stinging when the numbing medicine is injected. You may feel a pressure, a popping sensation, and discomfort when the procedure needle is inserted. Tell the person doing the test if you feel pain or numbness down your leg during the procedure. You may have back discomfort for several days after the procedure.

Synovial fluid:

During an arthrocentesis, a local anesthetic is given to numb the procedure area. You may feel mild discomfort or stinging when the numbing medicine is injected. You may feel pressure or discomfort during the procedure. Brief pain may be felt as the needle passes through the joint membrane. Your procedure site may be sore for several days.

Urine:

This test usually causes no discomfort.

Other body fluid or tissue sample:

A different sample other than the samples listed above may be used for this test. Instructions for what to do after a collection of other body fluid or tissue samples may vary. Ask the healthcare worker to instruct you on what to expect after this test is completed. If you have questions or concerns about what to expect after the test is completed, talk to the healthcare worker.

What should I do after the test?

Blood:

After a blood sample is collected from your vein, a bandage, cotton ball, or gauze may be placed on the area where the needle was inserted. You may be asked to apply pressure to the area. Avoid strenuous exercise immediately after your blood draw. Contact your healthcare worker if you feel pain or see redness, swelling, or discharge from the puncture site.

Cerebrospinal fluid:

After the lumbar puncture is complete, a bandage will be placed over the site and pressure held until the bleeding or drainage has stopped. You will need to lie flat for at least 1 to 2 hours after the lumbar puncture. Healthcare workers will monitor for drainage from the puncture site for a period of time after the test. You may be able carefully turn from your back to your side. You will be offered fluids to drink.

Synovial fluid:

After an arthrocentesis is completed, pressure may be held to the site, and a bandage secured over the puncture site. If a large amount of fluid is removed, an elastic wrap may be used to support your joint. To treat swelling and pain, you may place cold packs over the joint. Avoid heavy use of the joint for a few days. If you have difficulty using the affected arm or leg after the procedure, contact your healthcare worker.

You will be given instructions for how to care for your bandage and the signs and symptoms of infection to watch for. Contact your healthcare worker if you have a fever or increased pain, and if you see increasing redness, swelling, or pus at the procedure site.

Urine:

After collecting a urine sample, close the container if it has a lid. Place the container where the healthcare worker asked you to put it. Clean your hands with soap and water.

Other body fluid or tissue sample:

A different sample other than the samples listed above may be used for this test. Instructions for what to do after a collection of other body fluid or tissue samples may vary. Ask the healthcare worker to instruct you on what to expect after this test is completed. If you have questions or concerns about what to expect after the test is completed, talk to the healthcare worker.

What are the risks?

Blood: During a blood draw, a hematoma (blood-filled bump under the skin) or slight bleeding from the puncture site may occur. After a blood draw, a bruise or infection may occur at the puncture site. The person doing this test may need to perform it more than once. Talk to your healthcare worker if you have any concerns about the risks of this test.

Cerebrospinal fluid: Cerebrospinal fluid is collected by a procedure called a lumbar puncture. The most common risk of a lumbar puncture is spinal fluid leakage from the puncture site. This procedure may cause a mild to severe headache, which may last for several days. Although rare, other risks include infection, nerve injury, bleeding in the spinal canal, and damage to the discs in between your spine. If you have a medical condition, or are using a medication or supplement that causes excessive bleeding, you are at a higher risk of bleeding from the puncture site. If you have a condition where you have increased pressure within your skull, such as a traumatic head injury or a large brain tumor, this procedure carries a risk of brain herniation (where the skull contents are pressed down on to the spinal cord causing brain damage). The person doing this test may need to perform it more than once. Talk to your healthcare worker if you have any concerns about the risks of this procedure.

Synovial fluid: An arthrocentesis is the procedure used to get a sample of synovial fluid for testing. Risks of an arthrocentesis include bleeding into the joint and joint infection. If you have a medical condition, or are using a medication or supplement that causes excessive bleeding, you are at a higher risk of bleeding from the puncture site. The person doing this procedure may need to perform it more than once. Talk to your healthcare worker if you have any concerns about the risks of having an arthrocentesis.

Urine: A urine test is generally considered safe. Talk to your healthcare worker if you have questions or concerns about this test.

Other body fluid or tissue samples: A different sample other than the samples listed above may be used for this test. Ask your healthcare worker to explain the risks of this test to you. If you have questions or concerns about this test, talk to the healthcare worker.

What are normal results for this test?

Laboratory test results may vary depending on your age, gender, health history, the method used for the test, and many other factors. If your results are different from the results suggested below, this may not mean that you have a disease. Contact your healthcare worker if you have any questions. The following is considered to be a normal result for this test:

  • Negative [4]

What follow up should I do after this test?

Ask your healthcare worker how you will be informed of the test results. You may be asked to call for results, schedule an appointment to discuss results, or notified of results by mail. Follow up care varies depending on many factors related to your test. Sometimes there is no follow up after you have been notified of test results. At other times follow up may be suggested or necessary. Some examples of follow up care include changes to medication or treatment plans, referral to a specialist, more or less frequent monitoring, and additional tests or procedures. Talk with your healthcare worker about any concerns or questions you have regarding follow up care or instructions.

Cerebrospinal fluid:

You may experience headaches for hours to days, or even weeks after a lumbar puncture. The headaches are usually worse when sitting or standing upright and are relieved by lying down. Headaches are often associated with nausea, a stiff neck, tinnitus (ringing in the ears), photophobia (abnormal sensitivity to or intolerance of light), vertigo (dizziness), and blurred vision. Contact your healthcare worker if your symptoms worsen.

Where can I get more information?

Related Companies

References

  1. Chmielewski T, Fiett J, Gniadkowski M, et al: Improvement in the laboratory recognition of lyme borreliosis with the combination of culture and PCR methods. Mol Diagn 2003; 7:155-162.
  2. Nowakowski J, Schwartz I, Liveris D, et al: Laboratory diagnostic techniques for patients with early Lyme disease.. Clin Infect Dis 2001; 33:2023-2027.
  3. Henry JB: Clinical Diagnosis and Management by Laboratory Methods, 20th ed. Saunders, 2001.
  4. Lebech AM: Polymerase chain reaction in diagnosis of borrelia burgdorferi infections and studies on taxonomic classification. APMIS 2002; 110 Suppl. 105:7-40.

About This Site   |   Disclaimer   |  Privacy   |   Accessibility   |   Donations   |   Site Map
171 Ashley Avenue, Charleston, SC 29403 1.843.792.1414 | © 2014 Medical University of South Carolina

mobile web site iconrss feed iconText Messaging iconPodcast Library